Genomic regions associated with common root rot resistance in the barley variety Delta

Common root rot (CRR) caused by Bipolaris sorokiniana is a serious disease constraint in the dry temperate cereal growing regions of the world. Currently little is known about the genetic control of resistance to CRR in cereals. In this study based on a Delta/Lindwall barley population we have undertaken a bulked segregant analysis (BSA) and whole genome mapping approach utilising Diversity Arrays Technology (DArT) to identified quantitative trait loci (QTL) associated with CRR expression. One QTL each was identified on chromosomes 4HL and 5HL explaining 12 and 11% of the phenotypic variance, respectively

Genetic analysis of wheat rust resistance genes segregating in a Kariega x Avocet S population

Item does not contain fulltext14 mei 201

Mapping spot blotch resistance genes in four barley populations

Bipolaris sorokiniana (teleomorph: Cochliobolus sativus) is the fungal pathogen responsible for spot blotch in barley (Hordeum vulgare L.) and occurs worldwide in warmer, humid growing conditions. Current Australian barley varieties are largely susceptible to this disease and attempts are being made to introduce sources of resistance from North America. In this study we have compared chromosomal locations of spot blotch resistance reactions in four North American two-rowed barley lines; the North Dakota lines ND11231-12 and ND11231-11 and the Canadian lines TR251 and WPG8412-9-2-1. Diversity Arrays Technology (DArT)-based PCR, expressed sequence tag (EST) and SSR markers have been mapped across four populations derived from crosses between susceptible parental lines and these four resistant parents to determine the location of resistance loci. Quantitative trait loci (QTL) conferring resistance to spot blotch in adult plants (APR) were detected on chromosomes 3HS and 7HS. In contrast, seedling resistance (SLR) was controlled solely by a locus on chromosome 7HS. The phenotypic variance explained by the APR QTL on 3HS was between 16 and 25% and the phenotypic variance explained by the 7HS APR QTL was between 8 and 42% across the four populations. The SLR QTL on 7HS explained between 52 to 64% of the phenotypic variance. An examination of the pedigrees of these resistance sources supports the common identity of resistance in these lines and indicates that only a limited number of major resistance loci are available in current two-rowed germplasm

Validation of black point QTLs in wheat

Dissertação de mestrado em Psicologia (Psicologia da Educação, Desenvolvimento e Aconselhamento), apresentada à Faculdade de Psicologia e de Ciências da Educação da Universidade de CoimbraO presente estudo tem como objetivo compreender que tipo de experiências subjetivas as pessoas procuram quando consomem alguma droga e quais são, na sua perspetiva, as mais desejáveis, através da utilização da escala Drug Experiences Inventory (Redmon, 2005). Mais especificamente, pretende-se avaliar se a experiência subjetiva inerente ao consumo de substâncias constitui uma dimensão relevante a ter em consideração na definição de estratégias de prevenção e intervenção à dependência às drogas. Para tanto, recorreu-se a uma amostra de 208 participantes, sendo que 189 residem na região centro de Portugal e 19 provêm de serviços que prestam apoio a indivíduos que são ou foram dependentes de alguma substância. Através das análises efetuadas, conclui-se que os participantes usufruem de uma variedade de experiências quando estão sob o efeito de alguma droga e que discriminam essas mesmas experiências em função da desejabilidade que lhes atribuem. Os resultados obtidos revelam a importância de ser considerada a dimensão subjetiva do consumo de substâncias na prevenção e tratamento das dependências.The aim of this study is to understand what kind of subjective experiences people seek when they consume drugs and what are the most desirable for them, using de scale Drug Experiences Inventory (Redmon, 2005). More specifically, we intended to evaluate if the subjective experience inherent in the substance use is a relevant dimension to consider in defining strategies for prevention and intervention for drug addiction. In order to arrive at such evaluation, we collected a sample of 208 participants, of wich 189 were part of the normal group, and 19 (clinical sample) were recruited in agencies that provide support services for individuals with addiction to some kind of drug. Through the analyses performed, it is concluded that the participantes enjoy a variety of experiences and they discriminate them in terms of desirability they attribute to them. The results reveal the importance of considering the subjective dimension of drug use in prevention and treatment of addiction

Chromosome composition in an F2 hexaploid x durum cross analysed by DArT markers and MCFISH

A major constraint to tetraploid durum wheat production in Australia is widespread susceptibility to crown rot, due to infection by Fusarium pseudograminearum